The analysis of endogenous small molecules in biological matrices such as plasma, urine or tissue is important in many clinical, biotechnological and nutritional studies. In a broad sense, studies requiring these analyzes can be divided into two classes.
The first class involves studies where the targets are known biomarkers of a biological process. In such a case the number of compounds is often limited and can be analyzed with a target LC-MS method or an immuno assay such as ELISA. The second class involves the analysis of a large number of various compounds, and is often applied in metabolomics studies that are aimed at the discovery of new biomarkers or drug targets.