Bisphenol A is a high production volume compound and used to produce polymers. Since several years, there has been controversy about the safety of Bisphenol A, related to its application in food contact materials such as baby bottles. Consequently, human exposure to Bisphenol A has been investigated in a number of biomonitoring studies.
However, accurate analysis of Bisphenol A at sub-ppb levels is a challenging task, since Bisphenol A is a widely used material and is ubiquitous in the environment at trace concentrations. Propensity for contamination of biological samples with Bisphenol A is reported in literature during sample collection, storage, and/or analysis. Contamination by trace levels of Bisphenol A is so pervasive that even with extraordinary care, it is difficult to completely exclude the introduction of bisphenol A into biological samples. Consequently, contamination might have an impact on Bisphenol A biomonitoring data.
In a recent study, TNO Triskelion demonstrated the feasibility of analyzing Bisphenol A in human urine at sub-ppb levels with acceptable recovery and repeatability, using a SPE-UPLC-MS/MS method, provided that sufficient measures are taken to avoid contamination throughout the procedure from sample collection to sample handling and analysis. The limit of quantification was 0.1 ng/ml for unconjugated BPA and 0.2 ng/ml for total BPA in human urine. The results of this study have been published in:
Quantitative analysis of unconjugated and total bisphenol A in human urine using solid-phase extraction and UPLC–MS/MS: Method implementation, method qualification and troubleshooting.
Journal of Chromatography B, Volume 1005, 15 November 2015, Pages 30–38.